看到朋友圈小伙伴转发了一个有味道的研究:研究者们收集了 12个中国成年人的尿液,we performed scRNA-seq on voided urine samples. 50–100 mL middle stream urine samples were collected from 12 Chinese healthy adults and combined for droplet-based single-cell RNA sequencing after flow cytometric sorting of live cells.这个droplet-based单细胞转录组技术呢,在文章的附件里面提到了,就是大名鼎鼎的 10x Chromium Controller (10x Genomics). ,获得了 2200个细胞的数据,但是经过质量控制后留下来1010个细胞走后面的降维聚类分群注释分析流程。只有一个10X样品数据,所以12个中国成年人的尿液是混合在一起的。质量控制也比较常规:We removed the low-quality cells with less than 200 or more than 5000 detected genes, or if their mitochondrial gene content was > 25%. Genes were filtered out that were detected in less than 3 cells.接下来就是常规的,普通的质控降
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