关于Medulloblastoma (MB)主要是有4个分子亚型:Wingless (WNT), Sonic hedgehog (SHH), Group 3, and Group 4转录组数据的标准分析这个研究者给出了如下所示的4张图:(A) Heatmap of differentially expressed genes in Olig2cKO versus GFAP-Ptch tumors.(B) Volcano plot of significantly altered genes (p (C) Gene set enrichment analysis (GSEA) of top differentially regulated genes.(D) GSEA plots of downregulated SHH and E2F1 pathway gene sets in Olig2cKO versus GFAP-Ptch tumors. 数据在 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120974 ,如下的4个测序样品:GSM3423064 RNA_GFAP_Ptch_1GSM3423065 RNA_GFAP_Ptch_2GSM3423066 RNA_GFAP_Ptch_Olig2_1GSM3423067 RNA_GFAP_Ptch_Olig2_2确实是非常标准的分析啦,差异分析后做出来火山图和热图,然后进行GSEA分析拿到一些生物学功能通路展示一下。但是很明显
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