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Agilent的芯片同样也是扫描得到图片，然后图像处理（主要是Agilent Feature Extraction (AFE) 软件）得到信号值，但是值得注意的是这个时候有 两个信号值矩阵 ，分别是：the background matrix Eb as well as for the foreground matrix E. 比如我们从 ArrayExpress 数据库下载一个 E-MTAB-3017 数据集，来源于文章：Peripheral blood gene expression profiles in obese subjects without metabolic syndrome 代码如下： library (ArrayExpress) ## getAE download all raw data for this study ## ArrayExpress just donwload the expressionSet rawset = ArrayExpress( "E-MTAB-3017" ) # http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3017/ ## it's not a AffyBatch object , but a NChannelSet for this study . ## for this platform the element names: E, Eb # the background matrix E
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