在前面我们提到的使用barcode方法进行干细胞命运追踪的文章中，同样，我们继续围绕文章的英语进行学习。俗话说的话，坚持就是胜利，我们相信，只要我们每天把读到的文章仔细整理，一定可以有所收获。收获超乎我们的想象。第一段：To take advantage of available mutants expressing inducible Cre
recombinase in specific cell types or developmental stages, we designed
a DNA cassette, Polylox, for endogenous barcoding. Polylox is composed of ten loxP sites in alternating orientations spaced 178 base
pairs (bp) apart11, thus allowing excisions and inversions (Fig. 1a).
The intervening nine DNA blocks have unique sequences based on
the AT2G21770 gene from Arabidopsis thaliana, serving as the barcode
‘alphabet’ (Fig. 1a; Supplementary Data). We reasoned that random par-
tial reco
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